4E78

Crystal structure of a product state assembly of HCV NS5B genotype 2a JFH-1 isolate with beta hairpin loop deletion bound to primer-template RNA with 3'-dG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	T, P	5'-R(*U*AP*CP*CP*GP*(GDO))-3'	polymer	6	1875.2	2
2	A	PROTEIN (RNA-directed RNA polymerase)	polymer	572	63736.2	1	UniProt (Q99IB8) Pfam (PF00998) In PDB	Hepatitis C virus (HCV)	NS5B, p68
3		water	water		18.0	37	Chemie (HOH)

Sequence modifications

A: 1 - 443 (UniProt: Q99IB8)

PDB	External Database	Details
Met -1	-	expression tag
Ser 0	-	expression tag
Gln 86	Glu 2528	engineered mutation
Gln 87	Glu 2529	engineered mutation

A: 454 - 570 (UniProt: Q99IB8)

PDB	External Database	Details
Gly 444	-	linker
Gly 445	-	linker
Leu 571	-	expression tag
Glu 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67486.6
All*	Total formula weight	67486.6

*Water molecules are not included.