4E57

Crystal Structure of spacer 6aa-shortened cephalosporin acylase mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cephalosporin acylase	polymer	698	77659.3	2	UniProt (O86089) Pfam (PF01804)	Pseudomonas sp.
2	AA, BA, C, CA, D... (B, A)	CADMIUM ION	non-polymer		112.4	33	Chemie (CD)
3	JA, KA (A, B)	water	water		18.0	1324	Chemie (HOH)

Sequence modifications

A, B: 4 - 686 (UniProt: O86089)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ile 3	-	expression tag
Glu 127	Asp 154	SEE REMARK 999
-	Pro 192	deletion
-	Asp 193	deletion
-	Leu 194	deletion
-	Ala 195	deletion
-	Asp 196	deletion
-	Gln 197	deletion
Ala 166	Ser 199	engineered mutation
Leu 687	-	expression tag
Ala 688	-	expression tag
Ala 689	-	expression tag
Ala 690	-	expression tag
Leu 691	-	expression tag
Glu 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	155318.6
Non-Polymers*	Number of molecules	33
	Total formula weight	3709.6
All*	Total formula weight	159028.1

*Water molecules are not included.