4E2P

Crystal Structure of a Post-tailoring Hydroxylase (HmtN) Involved in the Himastatin Biosynthesis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cytochrome P450 107B1 (P450CVIIB1)	polymer	419	47109.6	1	UniProt (D9WMQ6)	Streptomyces himastatinicus	P450-like enzyme, post-tailoring hydroxylase
2	B (A)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	C, D, E, F (A)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G (A)	water	water		18.0	219	Chemie (HOH)

Sequence modifications

A: 1 - 399 (UniProt: D9WMQ6)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47109.6
Non-Polymers*	Number of molecules	5
	Total formula weight	713.7
All*	Total formula weight	47823.3

*Water molecules are not included.