4E2P

Crystal Structure of a Post-tailoring Hydroxylase (HmtN) Involved in the Himastatin Biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	77.15
Detector technology	CCD
Collection date	2012-02-22
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.541870
Spacegroup name	P 21 21 21
Unit cell lengths	56.140, 72.290, 103.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.380 - 2.360
R-factor	0.24121
Rwork	0.239
R-free	0.28012
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jjn
RMSD bond length	0.005
RMSD bond angle	1.458
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.380	2.418
High resolution limit [Å]	2.360	2.358
Number of reflections	16424
Completeness [%]	96.5	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	10% PEG 3000, 0.2M magnesium chloride, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K