4DSI
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA, Se-dGTP and Calcium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 581 | 66245.9 | 1 | UniProt (D9N168) Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (E1C9K5) | Geobacillus stearothermophilus | |
| 2 | B (B) | DNA | polymer | 12 | 3687.4 | 1 | |||
| 3 | C (C) | Se-dGTP | polymer | 9 | 2691.8 | 1 | |||
| 4 | D, E, F, G, H (A) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 5 | I, J, K (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 6 | L (C) | 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (DGP) | |||
| 7 | M (C) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 8 | N, O, P (A, B, C) | water | water | 18.0 | 242 | Chemie (HOH) |
Sequence modifications
A: 298 - 876 (UniProt: D9N168)
| PDB | External Database | Details |
|---|---|---|
| Gly 296 | - | expression tag |
| Ser 297 | - | expression tag |
| Asp 598 | Ala 301 | engineered mutation |
| Val 713 | Pro 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 72625.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1136.0 | |
| All* | Total formula weight | 73761.0 |
