4DQQ

Ternary complex of Bacillus DNA Polymerase I Large Fragment E658A, DNA duplex, and rCTP (paired with dG of template) in presence of Mg2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	DNA polymerase	polymer	592	67416.3	2	UniProt (Q5KWC1) Pfam (PF22619) Pfam (PF00476)	Geobacillus kaustophilus
2	C, E (B, E)	DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DOC))-3')	polymer	9	2635.8	2
3	D, F (C, F)	DNA (5'-D(*CP*AP*TP*GP*GP*GP*AP*GP*TP*CP*AP*GP*G)-3')	polymer	13	4056.6	2
4	G, L (A, D)	CYTIDINE-5'-TRIPHOSPHATE	non-polymer		483.2	2	Chemie (CTP)
5	H, N (A, D)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	I, J, O, P (A, D)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	4	Chemie (MPD)
7	K (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
8	M (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
9	Q, R, S, T, U... (A, D, B, C, E...)	water	water		18.0	1233	Chemie (HOH)

Sequence modifications

A, D: 285 - 876 (UniProt: Q5KWC1)

PDB	External Database	Details
Ala 598	Asp 600	engineered mutation
Ala 658	Glu 660	engineered mutation
His 823	Arg 825	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148217.3
Non-Polymers*	Number of molecules	10
	Total formula weight	1850.7
All*	Total formula weight	150068.0

*Water molecules are not included.