4DQI

Ternary complex of Bacillus DNA Polymerase I Large Fragment, DNA duplex, and dCTP (paired with dG of template)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	DNA polymerase I	polymer	592	67474.3	2	UniProt (Q5KWC1) Pfam (PF22619) Pfam (PF00476) In PDB	Geobacillus kaustophilus
2	B, E	DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DOC))-3')	polymer	9	2635.8	2		Synthetic construct
3	C, F	DNA (5'-D(*C*AP*TP*GP*GP*GP*AP*GP*TP*CP*AP*GP*G)-3')	polymer	13	4056.6	2		Synthetic construct
4	A, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A, D	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	3	Chemie (DCP)
6	A, D	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7		water	water		18.0	1636	Chemie (HOH)

Sequence modifications

A, D: 285 - 876 (UniProt: Q5KWC1)

PDB	External Database	Details
Ala 598	Asp 600	engineered mutation
His 823	Arg 825	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148333.4
Non-Polymers*	Number of molecules	7
	Total formula weight	1642.2
All*	Total formula weight	149975.6

*Water molecules are not included.