4DNO

Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the 1,2-epoxyhexane hydrolysis intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative hydrolase	polymer	301	34163.7	4	UniProt (Q02P97) Pfam (PF12697) UniProt (by SIFTS) (A0A0H2ZD27)	Pseudomonas aeruginosa
2	E, F, G, H (A, B, C, D)	(2R)-hexane-1,2-diol	non-polymer		118.2	4	Chemie (7FB)
3	I, J, K, L (A, B, C, D)	water	water		18.0	995	Chemie (HOH)

Sequence modifications

A, B, C, D: 25 - 319 (UniProt: Q02P97)

PDB	External Database	Details
Gln 153	Glu 153	engineered mutation
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136654.9
Non-Polymers*	Number of molecules	4
	Total formula weight	472.7
All*	Total formula weight	137127.6

*Water molecules are not included.