4DGW

Crystal Structure of the SF3a splicing factor complex of U2 snRNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Pre-mRNA-splicing factor PRP9	polymer	402	48184.2	1	UniProt (P19736) Pfam (PF16958) Pfam (PF16837) Pfam (PF12171)	Saccharomyces cerevisiae (yeast)
2	B (B)	Pre-mRNA-splicing factor PRP21	polymer	152	18397.3	1	UniProt (P32524) Pfam (PF01805) Pfam (PF12230)	Saccharomyces cerevisiae (yeast)
3	C (C)	Pre-mRNA-splicing factor PRP11	polymer	231	23078.6	1	UniProt (Q07350) Pfam (PF16835)	Saccharomyces cerevisiae (yeast)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E, F (A, B)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 1 - 389 (UniProt: P19736)

PDB	External Database	Details
Gly -12	-	expression tag
Ser -11	-	expression tag
Pro -10	-	expression tag
Gly -9	-	expression tag
Ile -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ile -2	-	expression tag
Leu -1	-	expression tag
Glu 0	-	expression tag

B: 87 - 237 (UniProt: P32524)

PDB	External Database	Details
Mse 86	-	expression tag

C: 50 - 266 (UniProt: Q07350)

PDB	External Database	Details
Unk 36	-	SEE REAMRK 999
Unk 37	-	SEE REAMRK 999
Unk 38	-	SEE REAMRK 999
Unk 39	-	SEE REAMRK 999
Unk 40	-	SEE REAMRK 999
Unk 41	-	SEE REAMRK 999
Unk 42	-	SEE REAMRK 999
Unk 43	-	SEE REAMRK 999
Unk 44	-	SEE REAMRK 999
Unk 45	-	SEE REAMRK 999
Unk 46	-	SEE REAMRK 999
Unk 47	-	SEE REAMRK 999
Unk 48	-	SEE REAMRK 999
Unk 49	-	SEE REAMRK 999
Mse 153	Leu 153	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	89660.0
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	89725.5

*Water molecules are not included.