4DGW

Crystal Structure of the SF3a splicing factor complex of U2 snRNP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791, 0.9794, 0.9600
Spacegroup name	I 21 21 21
Unit cell lengths	100.781, 127.259, 169.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.863 - 3.112
R-factor	0.2329
Rwork	0.228
R-free	0.27840
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.625
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	19171
Completeness [%]	96.6	83
Redundancy		4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290	8-12% Ethanol, 100mM MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	6	290	8-12% Ethanol, 100mM MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K