4DFB

Crystal structure of aminoglycoside phosphotransferase aph(2")-id/aph(2")-iva in complex with kanamycin

Replaces:  3R81

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	APH(2")-Id	polymer	322	38279.0	2	UniProt (O68183) Pfam (PF01636)	Enterococcus casseliflavus	APH(2")-IVa
2	C, E (A, B)	KANAMYCIN A	non-polymer		484.5	2	Chemie (KAN)
3	D, F (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	G, H (A, B)	water	water		18.0	453	Chemie (HOH)

Sequence modifications

A, B: 1 - 301 (UniProt: O68183)

PDB	External Database	Details
Mse -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76558.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1039.9
All*	Total formula weight	77598.0

*Water molecules are not included.