4DEV

An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Acetyl-xylan esterase Est2A	polymer	408	46191.8	8	UniProt (E0RVY7) Pfam (PF00657)	Butyrivibrio proteoclasticus
2	BA, CA, DA, EA, FA... (F, G, A, H, B...)	ACETIC ACID	non-polymer		60.1	20	Chemie (ACY)
3	AA, GA, HA, K, LA... (E, G, A, H, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	7	Chemie (PEG)
4	L (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	IA, Q (G, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	MA, NA, OA, PA, QA... (A, B, C, D, E...)	water	water		18.0	1707	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 376 (UniProt: E0RVY7)

PDB	External Database	Details
Met -31	-	expression tag
Ser -30	-	expression tag
Tyr -29	-	expression tag
Tyr -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
Leu -21	-	expression tag
Glu -20	-	expression tag
Ser -19	-	expression tag
Thr -18	-	expression tag
Ser -17	-	expression tag
Leu -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Lys -13	-	expression tag
Ala -12	-	expression tag
Gly -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Ala 351	His 351	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	369534.3
Non-Polymers*	Number of molecules	30
	Total formula weight	2163.5
All*	Total formula weight	371697.8

*Water molecules are not included.