4DBZ

Crystal Structure of V151L Actinorhodin Polyketide Ketoreductase with NADPH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ketoacyl reductase	polymer	281	29478.4	2	UniProt (P16544) Pfam (PF00106) In PDB	Streptomyces coelicolor
2	A, B	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	2	Chemie (NDP)
3		water	water		18.0	44	Chemie (HOH)

Sequence modifications

A, B: 1 - 261 (UniProt: P16544)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 151	Val 151	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58956.8
Non-Polymers*	Number of molecules	2
	Total formula weight	1490.8
All*	Total formula weight	60447.6

*Water molecules are not included.