4DBL

Crystal structure of E159Q mutant of BtuCDF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, F, G (A, B, F, G)	Vitamin B12 import system permease protein BtuC	polymer	349	37650.6	4	UniProt (P06609) Pfam (PF01032)	Escherichia coli
2	C, D, H, I (C, D, H, I)	Vitamin B12 import ATP-binding protein BtuD	polymer	249	27097.1	4	UniProt (P06611) Pfam (PF00005)	Escherichia coli	Vitamin B12-transporting ATPase
3	E, J (E, J)	Vitamin B12-binding protein	polymer	255	28238.1	2	UniProt (P37028) Pfam (PF01497)	Escherichia coli
4	K, O, S, W (C, D, H, I)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
5	L, M, N, P, Q... (C, D, H, I)	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)

Sequence modifications

A, B, F, G: 1 - 326 (UniProt: P06609)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ser 18	Cys 18	engineered mutation
Ser 32	Cys 32	engineered mutation
Ser 120	Cys 120	engineered mutation
Ser 156	Cys 156	engineered mutation
Ser 205	Cys 205	engineered mutation
Ser 206	Cys 206	engineered mutation
Ser 267	Cys 267	engineered mutation

C, D, H, I: 1 - 249 (UniProt: P06611)

PDB	External Database	Details
Gln 159	Glu 159	engineered mutation
Ser 180	Cys 180	engineered mutation

E, J: 22 - 266 (UniProt: P37028)

PDB	External Database	Details
Met 21	-	initiating methionine
Ser 267	-	expression tag
Gly 268	-	expression tag
Ser 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	315467.2
Non-Polymers*	Number of molecules	16
	Total formula weight	1532.6
All*	Total formula weight	316999.8

*Water molecules are not included.