4DBL
Crystal structure of E159Q mutant of BtuCDF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-11-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 133.650, 166.930, 132.820 |
Unit cell angles | 90.00, 119.76, 90.00 |
Refinement procedure
Resolution | 30.015 - 3.493 |
R-factor | 0.2153 |
Rwork | 0.214 |
R-free | 0.25130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.784 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7.2_869) |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.580 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 59380 | |
<I/σ(I)> | 1.957 | |
Completeness [%] | 93.1 | 88.2 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 0.1M sodium citrate, pH 5.4, 0.3-0.4M ammonium sulphate, 30-35% PEG-400, VAPOR DIFFUSION, SITTING DROP, temperature 293K |