4D7Q

Crystal structure of a chimeric protein with the Sec7 domain of Legionella pneumophila RalF and the capping domain of Rickettsia prowazekii RalF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RALF, PROLINE/BETAINE TRANSPORTER	polymer	352	40515.7	1	UniProt (Q8RT31) UniProt (M9TGB4) Pfam (PF18248) UniProt (by SIFTS) (Q9ZDF5)	LEGIONELLA PNEUMOPHILA
2	B (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
3	C (A)	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)
4	D, E, F, G (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	H, I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
6	J (A)	water	water		18.0	268	Chemie (HOH)

Sequence modifications

A: 2 - 190 (UniProt: Q8RT31)

PDB	External Database	Details
Met -5	-	expression tag
Lys -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Tyr 160	Asp 160	engineered mutation
Lys 339	-	expression tag
Ser 340	-	expression tag
Gln 341	-	expression tag
Tyr 342	-	expression tag
Asp 343	-	expression tag
Asn 344	-	expression tag
Ile 345	-	expression tag
Arg 346	-	expression tag

A: 191 - 338 (UniProt: M9TGB4)

PDB	External Database	Details
Glu 193	Lys 191	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40515.7
Non-Polymers*	Number of molecules	8
	Total formula weight	880.9
All*	Total formula weight	41396.6

*Water molecules are not included.