4D7Q
Crystal structure of a chimeric protein with the Sec7 domain of Legionella pneumophila RalF and the capping domain of Rickettsia prowazekii RalF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.310, 77.440, 119.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.720 - 2.000 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.720 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.900 |
| Number of reflections | 25893 | |
| <I/σ(I)> | 8.07 | 1.47 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 4.38 | 4.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 0.2 M POTASSIUM NITRATE, 0.1 M HEPES PH 6.8-7.8, 15-25% PEG 3350 |
