4D25
Crystal structure of the Bombyx mori Vasa helicase (E339Q) in complex with RNA and AMPPNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | BMVLG PROTEIN | polymer | 434 | 48129.1 | 1 | UniProt (O01378) Pfam (PF00270) Pfam (PF00271) | BOMBYX MORI (DOMESTIC SILKWORM) | BM VASA |
| 2 | B (D) | 5'-R(*UP*GP*AP*CP*AP*UP)-3' | polymer | 6 | 1876.2 | 1 | BOMBYX MORI | ||
| 3 | C (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (D) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | F, G (A, D) | water | water | 18.0 | 330 | Chemie (HOH) |
Sequence modifications
A: 135 - 564 (UniProt: O01378)
| PDB | External Database | Details |
|---|---|---|
| Gly 131 | - | expression tag |
| Ala 132 | - | expression tag |
| Met 133 | - | expression tag |
| Gly 134 | - | expression tag |
| Gln 339 | Glu 339 | engineered mutation |
| Cys 342 | Arg 342 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 50005.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 622.6 | |
| All* | Total formula weight | 50627.8 |
