4D25
Crystal structure of the Bombyx mori Vasa helicase (E339Q) in complex with RNA and AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.480, 78.570, 104.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.910 - 1.900 |
| R-factor | 0.17327 |
| Rwork | 0.171 |
| R-free | 0.20837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2db3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.520 |
| Number of reflections | 34183 | |
| <I/σ(I)> | 10.9 | 2.7 |
| Completeness [%] | 98.1 | 98.4 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES, PH 7.5 AND 25% PEG 3350 |
