4CTE

Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENDO-1,3-BETA-GLUCANASE, FAMILY GH16	polymer	233	26234.7	2	UniProt (G0L2L9) Pfam (PF00722)	ZOBELLIA GALACTANIVORANS	LAMINARINASE
2	C (C)	1-thio-beta-D-glucopyranose-(1-3)-1-thio-beta-D-glucopyranose	branched		374.4	1	In PDB GlyTouCan (G90274QA)
3	D (D)	beta-D-glucopyranose-(1-3)-1-thio-beta-D-glucopyranose-(1-3)-1-thio-beta-D-glucopyranose	branched		536.6	1	In PDB GlyTouCan (G21627PG)
4	E, I (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F, J (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	G, L (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	H, M (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	K (B)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
9	N (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
10	O, P (A, B)	water	water		18.0	333	Chemie (HOH)

Sequence modifications

A, B: 23 - 255 (UniProt: G0L2L9)

PDB	External Database	Details
Ser 142	Glu 142	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52469.4
Branched	Number of molecules	2
	Total formula weight	911.0
Non-Polymers*	Number of molecules	10
	Total formula weight	541.4
All*	Total formula weight	53921.8

*Water molecules are not included.