4CTE
Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.010, 93.930, 142.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.110 - 1.800 |
| R-factor | 0.17946 |
| Rwork | 0.177 |
| R-free | 0.22221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4crq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.986 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.070 | 0.690 |
| Number of reflections | 72254 | |
| <I/σ(I)> | 16.8 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 11% PEG 6000, 100 MM NA ACETATE PH 5.0, 220 MM MGCL2, 4% ISOPROPANOL AND 3% GLYCEROL |
