4CS3
Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with an adenylated furan-bearing noncanonical amino acid and pyrophosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PYRROLYSINE--TRNA LIGASE | polymer | 274 | 31736.4 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | METHANOSARCINA MAZEI | PYRROLYSINE--TRNA(PYL) LIGASE, PYRROLYSYL-TRNA SYNTHETASE, PYLRS |
| 2 | B (A) | LYSINE | non-polymer | 147.2 | 1 | Chemie (LYS) | |||
| 3 | C (A) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) | |||
| 4 | D (A) | 2-(furan-2-yl)ethyl hydrogen carbonate | non-polymer | 156.1 | 1 | Chemie (FU0) | |||
| 5 | E (A) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) | |||
| 6 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | H (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 8 | I (A) | water | water | 18.0 | 250 | Chemie (HOH) |
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Met 187 | - | expression tag |
| Ala 306 | Tyr 306 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
| His 455 | - | expression tag |
| His 456 | - | expression tag |
| His 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31736.4 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 937.2 | |
| All* | Total formula weight | 32673.6 |
