4CS3
Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with an adenylated furan-bearing noncanonical amino acid and pyrophosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PYRROLYSINE--TRNA LIGASE | polymer | 274 | 31736.4 | 1 | UniProt (Q8PWY1) | METHANOSARCINA MAZEI | PYRROLYSINE--TRNA(PYL) LIGASE, PYRROLYSYL-TRNA SYNTHETASE, PYLRS |
| 2 | B (A) | LYSINE | non-polymer | 147.2 | 1 | Chemie (LYS) PubChem (4477687) PubChem (641599) | |||
| 3 | C (A) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (224) PubChem (40467049) PubChem (40469468) PubChem (34768) PubChem (44348067) PubChem (83862) PubChem (45358029) PubChem (46874558) PubChem (40459109) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (46875232) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (5316444) PubChem (92144436) PubChem (5742864) PubChem (122197642) PubChem (12358354) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 4 | D (A) | 2-(furan-2-yl)ethyl hydrogen carbonate | non-polymer | 156.1 | 1 | Chemie (FU0) PubChem (137349371) | |||
| 5 | E (A) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) PubChem (4995) | |||
| 6 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 7 | H (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 8 | I (A) | water | water | 18.0 | 250 | Chemie (HOH) |
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Met 187 | - | expression tag |
| Ala 306 | Tyr 306 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
| His 455 | - | expression tag |
| His 456 | - | expression tag |
| His 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31736.4 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 937.2 | |
| All* | Total formula weight | 32673.6 |
