4CS2
Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in its apo form
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PYRROLYSINE--TRNA LIGASE | polymer | 274 | 31736.4 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | METHANOSARCINA MAZEI | PYRROLYSINE--TRNA(PYL) LIGASE, PYRROLYSYL-TRNA SYNTHETASE, PYLRS, PYRROLYSYL-TRNA SYNTHETASE |
| 2 | B (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 3 | C, D, E, F (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 4 | G (A) | water | water | 18.0 | 126 | Chemie (HOH) |
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Met 187 | - | expression tag |
| His 455 | - | expression tag |
| His 456 | - | expression tag |
| His 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
| Ala 306 | Tyr 306 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31736.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 354.4 | |
| All* | Total formula weight | 32090.8 |
