4CKQ

X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CARBOHYDRATE BINDING FAMILY 6	polymer	409	46454.1	1	UniProt (C7HEF6) Pfam (PF17189) UniProt (by SIFTS) (A3DJS9) In PDB	CLOSTRIDIUM THERMOCELLUM	XYN30A
2	C	4 HISTIDINES FROM PROTEOLYSED HIS-TAG	polymer	4	570.6	1		SYNTHETIC CONSTRUCT
3	A	MALONIC ACID	non-polymer		104.1	1	Chemie (MLA)
4		water	water		18.0	647	Chemie (HOH)

Sequence modifications

A: 1 - 386 (UniProt: C7HEF6)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ser 77	Phe 110	engineered mutation
Cys 315	Tyr 348	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47024.7
Non-Polymers*	Number of molecules	1
	Total formula weight	104.1
All*	Total formula weight	47128.7

*Water molecules are not included.