4CKQ
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.090, 47.463, 53.620 |
| Unit cell angles | 83.15, 73.41, 65.87 |
Refinement procedure
| Resolution | 51.390 - 1.400 |
| R-factor | 0.14958 |
| Rwork | 0.148 |
| R-free | 0.17150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gtn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.837 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.930 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.200 | 0.860 |
| Number of reflections | 76088 | |
| <I/σ(I)> | 3.5 | 1.3 |
| Completeness [%] | 97.2 | 95.6 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M POTASSIUM SULFATE 20 % (W/V) POLYETHYLENE GLYCOL 3350 |
