4CK5
Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in the ADP state, based on cryo-electron microscopy experiment.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TUBULIN ALPHA-1D CHAIN | polymer | 452 | 50236.4 | 1 | UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) | BOS TAURUS (CATTLE) | ALPHA TUBULIN |
| 2 | B (B) | TUBULIN BETA-2B CHAIN | polymer | 445 | 49907.8 | 1 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | BOS TAURUS (CATTLE) | BETA TUBULIN |
| 3 | C (C) | KINESIN-LIKE PROTEIN KIF11 | polymer | 373 | 41673.1 | 1 | UniProt (P52732) Pfam (PF00225) | HOMO SAPIENS (HUMAN) | KINESIN-LIKE PROTEIN 1, KINESIN-LIKE SPINDLE PROTEIN HKSP, KINESIN-RELATED MOTOR PROTEIN EG5, THYROID RECEPTOR-INTERACTING PR OTEIN 5, TR-INTERACTING PROTEIN 5, TRIP-5, KINESIN-5 |
| 4 | D (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 5 | E (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 6 | F (B) | TAXOL | non-polymer | 853.9 | 1 | Chemie (TA1) | |||
| 7 | G (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | H (C) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
A: 1 - 452 (UniProt: Q2HJ86)
B: 1 - 445 (UniProt: Q6B856)
C: 1 - 360 (UniProt: P52732)
| PDB | External Database | Details |
|---|---|---|
| Ile 7 | Val 7 | conflict |
| Ile 114 | Leu 114 | conflict |
| Ser 136 | Leu 136 | conflict |
| Val 137 | Ile 137 | conflict |
| Glu 358 | Asp 358 | conflict |
| Val 437 | Met 437 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 55 | Thr 55 | conflict |
| Val 170 | Met 170 | conflict |
| Ala 296 | Ser 296 | conflict |
| Val 316 | Ile 316 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 43 | Cys 43 | engineered mutation |
| Ala 87 | Cys 87 | engineered mutation |
| Ala 99 | Cys 99 | engineered mutation |
| Cys 126 | Thr 126 | engineered mutation |
| His 361 | - | expression tag |
| His 362 | - | expression tag |
| His 363 | - | expression tag |
| His 364 | - | expression tag |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 141817.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 2271.8 | |
| All* | Total formula weight | 144089.0 |
