4CK0

Crystal structure of the integral membrane diacylglycerol kinase - form 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	DIACYLGLYCEROL KINASE	polymer	130	14240.5	3	UniProt (P0ABN1) Pfam (PF01219) In PDB	ESCHERICHIA COLI	DAGK, DIGLYCERIDE KINASE, DGK, DIACYLGLYCEROL KINASE -DELTA 4
2	B	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	2	Chemie (OLC)
3	C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	C	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
5		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 121 (UniProt: P0ABN1)

PDB	External Database	Details
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Leu 0	-	expression tag
Cys 53	Ile 54	engineered mutation
Leu 70	Ile 71	engineered mutation
Leu 96	Met 97	engineered mutation
Asp 107	Val 108	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	42721.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1349.1
All*	Total formula weight	44070.7

*Water molecules are not included.