4CK0
Crystal structure of the integral membrane diacylglycerol kinase - form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.820, 72.820, 195.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.006 - 2.924 |
R-factor | 0.2495 |
Rwork | 0.248 |
R-free | 0.28090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ze3 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.563 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.020 | 3.000 |
High resolution limit [Å] | 2.920 | 2.920 |
Rmerge | 0.100 | 0.760 |
Number of reflections | 13563 | |
<I/σ(I)> | 9.9 | 2.5 |
Completeness [%] | 99.1 | 99.5 |
Redundancy | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 7-9%(V/V) 2-METHYL-2-4-PENTANEDIOL, 0.1 M SODIUM CHLORIDE, 0.1 M LITHIUM NITRATE, 0.1 M SODIUM CITRATE PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREE CELSIUS WITH THE MONOOLEIN AS THE HOSTING LIPID. CRYSTALS WERE SOAKED AT 4 DEGREE CELSIUS WITH 10 MM AMPPCP AND 60 MM MAGNESIUM IN THE CRYSTALLIZATION CONDITION FOR 2 H BEFORE HARVESTING. |