4CFR

Ca-bound S100A4 C3S, C81S, C86S and F45W mutant complexed with non- muscle myosin IIA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PROTEIN S100-A4	polymer	104	12018.7	2	UniProt (P26447) Pfam (PF01023) In PDB	HOMO SAPIENS (HUMAN)	CALVASCULIN, METASTASIN, PLACENTAL CALCIUM-BINDING PROTEIN, PROTEIN MTS1, S100 CALCIUM-BINDING PROTEIN A4, S100A4
2	Q	MYOSIN-9	polymer	45	5358.2	1	UniProt (P35579) In PDB	HOMO SAPIENS (HUMAN)	CELLULAR MYOSIN HEAVY CHAIN\,TYPE A, MYOSIN HEAVY CHAIN 9, MYOSIN HEAVY CHAIN\,NON-MUSCLE IIA, NON-MUSCLE MYOSIN HEAVY CHAIN A, NMMHC-A, NON-MUSCLE MYOSIN HEAVY CHAIN IIA, NMMHC II-A, NMMHC-I IA, MYOSIN HEAVY CHAIN, NON MUSCLE MYOSIN IIA
3	A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4		water	water		18.0	253	Chemie (HOH)

Sequence modifications

A, B: 1 - 101 (UniProt: P26447)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 3	Cys 3	engineered mutation
Ser 81	Cys 81	engineered mutation
Trp 45	Phe 45	engineered mutation
Ser 86	Cys 86	engineered mutation

Q: 1893 - 1937 (UniProt: P35579)

PDB	External Database	Details
Tyr 1893	Arg 1893	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	29395.5
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	29555.8

*Water molecules are not included.