4CEH

Crystal structure of AddAB with a forked DNA substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ATP-DEPENDENT HELICASE/NUCLEASE SUBUNIT A	polymer	1232	141218.8	1	UniProt (P23478) Pfam (PF00580) Pfam (PF13361) Pfam (PF12705) In PDB	BACILLUS SUBTILIS SUBSP. SUBTILIS STR. 168	ATP-DEPENDENT HELICASE/NUCLEASE ADDA, ATP-DEPENDENT HELICASE-NUCLEASE SUBUNIT A
2	B	ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B	polymer	1166	134769.4	1	UniProt (P23477) Pfam (PF21445) Pfam (PF13361) Pfam (PF12705) In PDB	BACILLUS SUBTILIS SUBSP. SUBTILIS STR. 168	ATP-DEPENDENT HELICASE/NUCLEASE ADDB, ATP-DEPENDENT HELICASE-NUCLEASE SUBUNIT B
3	X	DNA	polymer	65	19956.7	1		SYNTHETIC CONSTRUCT
4	B	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 1 - 1232 (UniProt: P23478)

PDB	External Database	Details
Gly 780	Ala 780	variant
Ala 1172	Asp 1172	engineered mutation

B: 1 - 1166 (UniProt: P23477)

PDB	External Database	Details
Asp 843	Glu 843	variant
Glu 844	Gln 844	variant
Ala 961	Asp 961	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	295945.0
Non-Polymers*	Number of molecules	1
	Total formula weight	351.6
All*	Total formula weight	296296.6

*Water molecules are not included.