4CEH
Crystal structure of AddAB with a forked DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.373, 96.766, 109.693 |
| Unit cell angles | 104.38, 96.11, 90.03 |
Refinement procedure
| Resolution | 29.939 - 3.240 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u44 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.616 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (PHASER-MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.520 | 3.350 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 47940 | |
| <I/σ(I)> | 13.2 | 2.3 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 0.1M TRIS-HCL PH 7.5, 15% PEG 4000, 0.8M SODIUM FORMATE, VAPOR DIFFUSION, HANGING DROP, 285K |
