4CEH
Crystal structure of AddAB with a forked DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 77.373, 96.766, 109.693 |
Unit cell angles | 104.38, 96.11, 90.03 |
Refinement procedure
Resolution | 29.939 - 3.240 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u44 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.616 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (PHASER-MR) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.520 | 3.350 |
High resolution limit [Å] | 3.240 | 3.240 |
Rmerge | 0.050 | 0.330 |
Number of reflections | 47940 | |
<I/σ(I)> | 13.2 | 2.3 |
Completeness [%] | 98.5 | 97.7 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 0.1M TRIS-HCL PH 7.5, 15% PEG 4000, 0.8M SODIUM FORMATE, VAPOR DIFFUSION, HANGING DROP, 285K |