4CCN

60S ribosomal protein L8 histidine hydroxylase (NO66 L299C/C300S) in complex with Mn(II), N-oxalylglycine (NOG) and 60S ribosomal protein L8 (RPL8 G220C) peptide fragment (complex-2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	BIFUNCTIONAL LYSINE-SPECIFIC DEMETHYLASE AND HISTIDYL-HYDROXYLASE NO66	polymer	467	53215.1	2	UniProt (Q9H6W3) Pfam (PF08007) Pfam (PF21233) In PDB	HOMO SAPIENS (HUMAN)	60S RIBOSOMAL PROTEIN L8 HISTIDINE HYDROXYLASE, HISTONE LYSINE DEMETHYLASE NO66, MYC-ASSOCIATED PROTEIN WITH JMJC DOMAIN, NUCLEOLAR PROTEIN 66, HSNO66, RIBOSOMAL OXYGENASE NO66, ROX
2	C, D	60S RIBOSOMAL PROTEIN L8	polymer	35	3739.3	2	UniProt (P62917) In PDB	HOMO SAPIENS
3	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	A, B	N-OXALYLGLYCINE	non-polymer		147.1	2	Chemie (OGA)
5	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7		water	water		18.0	363	Chemie (HOH)

Sequence modifications

A, B: 183 - 641 (UniProt: Q9H6W3)

PDB	External Database	Details
Met 182	-	expression tag
Ala 642	-	expression tag
Glu 643	-	expression tag
Asn 644	-	expression tag
Leu 645	-	expression tag
Tyr 646	-	expression tag
Phe 647	-	expression tag
Gln 648	-	expression tag
Cys 299	Leu 299	engineered mutation
Ser 300	Cys 300	engineered mutation
Ala 364	Val 364	engineered mutation

C, D: 205 - 239 (UniProt: P62917)

PDB	External Database	Details
Cys 220	Gly 220	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	113908.8
Non-Polymers*	Number of molecules	7
	Total formula weight	658.2
All*	Total formula weight	114567.0

*Water molecules are not included.