4BX7
trans-divalent streptavidin bound to biotin-4-fluorescein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | STREPTAVIDIN | polymer | 128 | 13321.4 | 1 | UniProt (P22629) Pfam (PF01382) | STREPTOMYCES AVIDINII | |
| 2 | B (B) | STREPTAVIDIN | polymer | 133 | 14056.0 | 1 | UniProt (P22629) Pfam (PF01382) | STREPTOMYCES AVIDINII | |
| 3 | C (A) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 4 | D (B) | biotin-4-fluorescein | non-polymer | 644.7 | 1 | Chemie (B4F) | |||
| 5 | E, F (A, B) | water | water | 18.0 | 78 | Chemie (HOH) |
Sequence modifications
A: 13 - 139 (UniProt: P22629)
B: 13 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Ala 140 | - | expression tag |
| Ala 23 | Asn 47 | engineered mutation |
| Asp 27 | Ser 51 | engineered mutation |
| Ala 45 | Ser 69 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Glu 140 | - | expression tag |
| Glu 141 | - | expression tag |
| Glu 142 | - | expression tag |
| Glu 143 | - | expression tag |
| Glu 144 | - | expression tag |
| Glu 145 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 27377.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 762.9 | |
| All* | Total formula weight | 28140.3 |
