4BX7
trans-divalent streptavidin bound to biotin-4-fluorescein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-07 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 64.410, 64.410, 103.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.064 - 2.260 |
R-factor | 0.1863 |
Rwork | 0.184 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ry1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.060 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.080 | 0.750 |
Number of reflections | 12059 | |
<I/σ(I)> | 18.95 | 3.26 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 9.6 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 65% V/V MPD, 0.1 M MES PH 4.0. CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOR-DIFFUSION METHOD AT 277 K, REACHED A MAXIMUM SIZE AFTER 14 DAYS AND WERE HARVESTED SOON AFTER |