4BV0

High Resolution Structure of Evolved Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NEUROTENSIN RECEPTOR TYPE 1	polymer	338	37719.2	2	UniProt (P20789) Pfam (PF00001)	RATTUS NORVEGICUS (NORWAY RAT)	NEUROTENSIN RECEPTOR 1 TM86VDIC3B, NT-R-1, NTR1, HIGH-AFFINITY LEVOCABASTINE-INSENSITIVE NEUROTENSIN RECEPTOR, NTRH
2	C, D (C, D)	NEUROTENSIN/NEUROMEDIN N	polymer	10	1087.3	2	UniProt (P20068)	RATTUS NORVEGICUS (NORWAY RAT)	LARGE NEUROMEDIN N, NMN-125, NEUROMEDIN N, NN, NMN, NEUROTENSIN, NT, TAIL PEPTIDE

Sequence modifications

A, B: 50 - 390 (UniProt: P20789)

PDB	External Database	Details
Gly 46	-	expression tag
Pro 47	-	expression tag
Gly 48	-	expression tag
Ser 49	-	expression tag
Gly 83	Ser 83	engineered mutation
Leu 86	Ala 86	engineered mutation
Arg 101	Thr 101	engineered mutation
Asp 103	His 103	engineered mutation
Tyr 105	His 105	engineered mutation
Phe 119	Leu 119	engineered mutation
Leu 121	Met 121	engineered mutation
Asp 124	Glu 124	engineered mutation
Val 125	Leu 125	engineered mutation
Lys 143	Arg 143	engineered mutation
Glu 150	Asp 150	engineered mutation
Val 161	Ala 161	engineered mutation
Leu 167	Arg 167	engineered mutation
Arg 172	Cys 172	engineered mutation
His 177	Ala 177	engineered mutation
Val 208	Met 208	engineered mutation
Leu 213	Arg 213	engineered mutation
Leu 234	Val 234	engineered mutation
Leu 240	Val 240	engineered mutation
Ala 253	Ile 253	engineered mutation
Arg 262	Asn 262	engineered mutation
Arg 263	Lys 263	engineered mutation
-	Val 280	deletion
-	Gly 281	deletion
-	Thr 282	deletion
-	His 283	deletion
-	Asn 284	deletion
-	Gly 285	deletion
-	Leu 286	deletion
-	Glu 287	deletion
-	His 288	deletion
-	Ser 289	deletion
-	Thr 290	deletion
-	Phe 291	deletion
-	Asn 292	deletion
-	Met 293	deletion
-	Thr 294	deletion
-	Ile 295	deletion
Arg 305	His 305	engineered mutation
Met 313	Val 313	engineered mutation
Val 332	Cys 332	engineered mutation
Ala 342	Phe 342	engineered mutation
Ser 354	Thr 354	engineered mutation
Val 358	Phe 358	engineered mutation
Ala 362	Ser 362	engineered mutation
Thr 391	-	expression tag
Arg 392	-	expression tag
Glu 393	-	expression tag
Leu 394	-	expression tag
Glu 395	-	expression tag
Val 396	-	expression tag
Leu 397	-	expression tag
Phe 398	-	expression tag
Gln 399	-	expression tag

C, D: 8 - 13 (UniProt: P20068)

PDB	External Database	Details
Gly 4	-	expression tag
Pro 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	77613.0
All*	Total formula weight	77613.0

*Water molecules are not included.