4BV0

High Resolution Structure of Evolved Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 21
Unit cell lengths	60.580, 91.560, 208.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 3.100
Rwork	0.284
R-free	0.31050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zev
RMSD bond length	0.003
RMSD bond angle	0.651
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.100
Number of reflections	21665
<I/σ(I)>	15.55	0.51
Completeness [%]	99.2	97
Redundancy	6.3	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.4		22.5% (V/V) PEG600, 0.5 M NACL, 50 MM GLYCINE PH 9.4