4BTH
The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ACYL-HOMOSERINE LACTONE ACYLASE PVDQ SUBUNIT ALPHA | polymer | 170 | 18666.0 | 1 | UniProt (Q9I194) | PSEUDOMONAS AERUGINOSA | AHL ACYLASE PVDQ, ACYL-HSL ACYLASE PVDQ, ACYL-HSL ACYLASE PVDQ SUBUNIT ALPHA, |
| 2 | B (B) | ACYL-HOMOSERINE LACTONE ACYLASE PVDQ SUBUNIT BETA | polymer | 546 | 60505.9 | 1 | UniProt (Q9I194) Pfam (PF01804) | PSEUDOMONAS AERUGINOSA | AHL ACYLASE PVDQ, ACYL-HSL ACYLASE PVDQ, ACYL-HSL ACYLASE PVDQ SUBUNIT BETA |
| 3 | C, D, E, F, G (A, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 4 | H, I (A, B) | water | water | 18.0 | 254 | Chemie (HOH) |
Sequence modifications
A: 1 - 170 (UniProt: Q9I194)
B: 1 - 546 (UniProt: Q9I194)
| PDB | External Database | Details |
|---|---|---|
| Trp 146 | Leu 169 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Tyr 24 | Phe 240 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 79171.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 460.5 | |
| All* | Total formula weight | 79632.4 |
