4BOW

Crystal structure of LamA_E269S from Z. galactanivorans in complex with laminaritriose and laminaritetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENDO-1,3-BETA-GLUCANASE, FAMILY GH16	polymer	256	29219.9	2	UniProt (G0L5X4) Pfam (PF00722)	ZOBELLIA GALACTANIVORANS	ENDO-BETA-1,3-GLUCANASE
2	C (C)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose	branched		504.4	1	In PDB GlyTouCan (G51422BS)
3	D (D)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		666.6	1	In PDB GlyTouCan (G87841AX)
4	E, G (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	H (B)	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
7	I, J (A, B)	water	water		18.0	545	Chemie (HOH)

Sequence modifications

A, B: 136 - 383 (UniProt: G0L5X4)

PDB	External Database	Details
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
Gly 134	-	expression tag
Ser 135	-	expression tag
Ser 269	Glu 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58439.8
Branched	Number of molecules	2
	Total formula weight	1171.0
Non-Polymers*	Number of molecules	4
	Total formula weight	283.3
All*	Total formula weight	59894.1

*Water molecules are not included.