4BLV

Crystal structure of Escherichia coli 23S rRNA (A2030-N6)- methyltransferase RlmJ in complex with S-adenosylmethionine (AdoMet)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE J	polymer	289	33088.9	2	UniProt (P37634) Pfam (PF04378)	ESCHERICHIA COLI
2	C, U (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
3	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	E, V (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	AA, BA, CA, DA, EA... (B, A)	1,2-ETHANEDIOL	non-polymer		62.1	27	Chemie (EDO)
6	S, T (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	KA, LA (A, B)	water	water		18.0	439	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: P37634)

PDB	External Database	Details
Ser 281	-	expression tag
Lys 282	-	expression tag
Gly 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66177.7
Non-Polymers*	Number of molecules	34
	Total formula weight	2969.2
All*	Total formula weight	69146.9

*Water molecules are not included.