4BIF

Biochemical and structural characterisation of a novel manganese- dependent hydroxynitrile lyase from bacteria

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	CUPIN 2 CONSERVED BARREL DOMAIN PROTEIN	polymer	156	17370.3	8	UniProt (E8WYN5) Pfam (PF07883) In PDB	GRANULICELLA TUNDRICOLA	GTHNL
2	A, B, C, D, E...	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3		water	water		18.0	707	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 131 (UniProt: E8WYN5)

PDB	External Database	Details
Met -24	-	expression tag
Ser -23	-	expression tag
Tyr -22	-	expression tag
Tyr -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	138962.1
Non-Polymers*	Number of molecules	8
	Total formula weight	439.5
All*	Total formula weight	139401.6

*Water molecules are not included.