4BIF

Biochemical and structural characterisation of a novel manganese- dependent hydroxynitrile lyase from bacteria

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016829	molecular_function	lyase activity
A	0046593	molecular_function	mandelonitrile lyase activity
A	0046872	molecular_function	metal ion binding
B	0016829	molecular_function	lyase activity
B	0046593	molecular_function	mandelonitrile lyase activity
B	0046872	molecular_function	metal ion binding
C	0016829	molecular_function	lyase activity
C	0046593	molecular_function	mandelonitrile lyase activity
C	0046872	molecular_function	metal ion binding
D	0016829	molecular_function	lyase activity
D	0046593	molecular_function	mandelonitrile lyase activity
D	0046872	molecular_function	metal ion binding
E	0016829	molecular_function	lyase activity
E	0046593	molecular_function	mandelonitrile lyase activity
E	0046872	molecular_function	metal ion binding
F	0016829	molecular_function	lyase activity
F	0046593	molecular_function	mandelonitrile lyase activity
F	0046872	molecular_function	metal ion binding
G	0016829	molecular_function	lyase activity
G	0046593	molecular_function	mandelonitrile lyase activity
G	0046872	molecular_function	metal ion binding
H	0016829	molecular_function	lyase activity
H	0046593	molecular_function	mandelonitrile lyase activity
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN A 1132

Chain	Residue
A	HIS53
A	HIS55
A	GLN59
A	HIS94
A	HIS96

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN B 1132

Chain	Residue
B	HIS96
B	HOH2057
B	HIS53
B	HIS55
B	GLN59
B	HIS94

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN C 1132

Chain	Residue
C	HIS53
C	HIS55
C	GLN59
C	HIS94
C	HIS96
C	HOH2052

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN D 1132

Chain	Residue
D	HIS53
D	HIS55
D	GLN59
D	HIS94
D	HIS96
D	HOH2049

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN E 1132

Chain	Residue
E	HIS53
E	HIS55
E	GLN59
E	HIS94
E	HIS96
E	HOH2061

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN F 1132

Chain	Residue
F	HIS53
F	HIS55
F	GLN59
F	HIS94
F	HIS96
F	HOH2062

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN G 1132

Chain	Residue
G	HIS53
G	HIS55
G	GLN59
G	HIS94
G	HIS96
G	HOH2031

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN H 1132

Chain	Residue
H	HIS53
H	HIS55
H	GLN59
H	HIS94
H	HIS96
H	HOH2040

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	496
Details	Domain: {"description":"Cupin type-2","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23981508","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2015","firstPage":"0","lastPage":"0","volume":"0","journal":"ChemCatChem","title":"Improving the Properties of Bacterial R-Selective Hydroxynitrile Lyases for Industrial Applications.","authors":["Wiedner R.","Kothbauer B.","Pavkov-Keller T.","Gruber-Khadjawi M.","Grube K.","Schwab H.","Steiner K."],"citationCrossReferences":[{"database":"DOI","id":"10.1002/CCTC.201402742"}]}},{"source":"PDB","id":"4BIF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4UXA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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