4B2A
Structure of the factor Xa-like trypsin variant triple-Ala (TGA) in complex with eglin C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | CATIONIC TRYPSIN | polymer | 223 | 23388.4 | 2 | UniProt (P00760) Pfam (PF00089) | BOS TAURUS (CATTLE) | BETA-TRYPSIN, ALPHA-TRYPSIN CHAIN 1, ALPHA-TRYPSIN CHAIN 2 |
| 2 | B, D (B, D) | EGLIN C | polymer | 66 | 7681.6 | 2 | UniProt (P01051) Pfam (PF00280) | HIRUDO MEDICINALIS (MEDICINAL LEECH) | |
| 3 | E, I (A, C) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | F, G, J (A, B, C) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | H (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 134 | Chemie (HOH) |
Sequence modifications
A, C: 16 - 245 (UniProt: P00760)
B, D: 5 - 70 (UniProt: P01051)
| PDB | External Database | Details |
|---|---|---|
| Glu 97 | Asn 102 | engineered mutation |
| Tyr 99 | Leu 104 | engineered mutation |
| Ser 172 | Tyr 175 | engineered mutation |
| Ser 173 | Pro 176 | engineered mutation |
| Phe 174 | Gly 177 | engineered mutation |
| Ile 175 | Gln 178 | engineered mutation |
| Glu 217 | Ser 218 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Lys 45 | Leu 45 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 62139.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 418.5 | |
| All* | Total formula weight | 62558.4 |
