4B1M

CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	LEVANASE	polymer	185	20643.7	3	UniProt (P05656) Pfam (PF06439)	BACILLUS SUBTILIS
2	D, E, F (D, E, F)	beta-D-fructofuranose-(2-6)-beta-D-fructofuranose	branched		342.3	3	In PDB BIRD (PRD_900051) GlyTouCan (G51829YA)		levanbiose
3	G, H, I, K (A, B, C)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	J (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	L, M, N (A, B, C)	water	water		18.0	469	Chemie (HOH)

Sequence modifications

A, B, C: 515 - 677 (UniProt: P05656)

PDB	External Database	Details
Met 493	-	expression tag
Gly 494	-	expression tag
His 495	-	expression tag
His 496	-	expression tag
His 497	-	expression tag
His 498	-	expression tag
His 499	-	expression tag
His 500	-	expression tag
His 501	-	expression tag
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
Ser 505	-	expression tag
Ser 506	-	expression tag
Gly 507	-	expression tag
His 508	-	expression tag
Ile 509	-	expression tag
Glu 510	-	expression tag
Gly 511	-	expression tag
Arg 512	-	expression tag
His 513	-	expression tag
Met 514	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	61931.1
Branched	Number of molecules	3
	Total formula weight	1026.9
Non-Polymers*	Number of molecules	5
	Total formula weight	188.0
All*	Total formula weight	63146.0

*Water molecules are not included.