4B1M
CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.897, 36.909, 87.355 |
Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
Resolution | 87.350 - 1.100 |
R-factor | 0.15759 |
Rwork | 0.156 |
R-free | 0.18121 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4azz |
RMSD bond length | 0.013 |
RMSD bond angle | 1.909 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.670 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.050 | 0.370 |
Number of reflections | 158032 | |
<I/σ(I)> | 12.5 | 2 |
Completeness [%] | 91.2 | 55.4 |
Redundancy | 3.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.6-2.2 M AMMONIUM SULFATE, 1.4 - 2.4 M NACL, 5-6 MM LEVAN TRIOSE |