4B0A

The high-resolution structure of yTBP-yTAF1 identifies conserved and competing interaction surfaces in transcriptional activation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1, LINKER, TATA-BOX-BINDING PROTEIN	polymer	278	30316.4	1	UniProt (P46677) UniProt (P13393) Pfam (PF00352) In PDB	SACCHAROMYCES CEREVISIAE	TATA SEQUENCE-BINDING PROTEIN, TBP, TATA-BINDING FACTOR, TATA-BOX FACTOR, TRANSCRIPTION FACTOR D, TRANSCRIPTION INITIATION FACTOR TFIID TBP SUBUNIT, TAFII-130, TAFII-145, TBP-ASSOCIATED FACTOR 1, TBP-ASSOCIATED FACTOR 145 KDA
2	A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
3	A	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	213	Chemie (HOH)

Sequence modifications

A: 8 - 71 (UniProt: P46677)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Leu 0	-	expression tag
Val 1	-	expression tag
Pro 2	-	expression tag
Arg 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
Met 7	-	expression tag
Gly 72	-	linker
Gly 73	-	linker
Gly 74	-	linker
Ser 75	-	linker
Gly 76	-	linker
Gly 77	-	linker
Gly 78	-	linker
Ser 79	-	linker
Gly 80	-	linker
Gly 81	-	linker
Gly 82	-	linker
Ser 83	-	linker
Met 84	-	linker

A: 161 - 340 (UniProt: P13393)

PDB	External Database	Details
Ala 41	Gly 41	engineered mutation
Arg 42	Ala 42	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30316.4
Non-Polymers*	Number of molecules	10
	Total formula weight	708.3
All*	Total formula weight	31024.6

*Water molecules are not included.