4B0A
The high-resolution structure of yTBP-yTAF1 identifies conserved and competing interaction surfaces in transcriptional activation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1, LINKER, TATA-BOX-BINDING PROTEIN | polymer | 278 | 30316.4 | 1 | UniProt (P46677) UniProt (P13393) Pfam (PF00352) | SACCHAROMYCES CEREVISIAE | TATA SEQUENCE-BINDING PROTEIN, TBP, TATA-BINDING FACTOR, TATA-BOX FACTOR, TRANSCRIPTION FACTOR D, TRANSCRIPTION INITIATION FACTOR TFIID TBP SUBUNIT, TAFII-130, TAFII-145, TBP-ASSOCIATED FACTOR 1, TBP-ASSOCIATED FACTOR 145 KDA |
| 2 | B, C, J (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 3 | D, E, F, G, H... (A) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 4 | K (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | L (A) | water | water | 18.0 | 213 | Chemie (HOH) |
Sequence modifications
A: 8 - 71 (UniProt: P46677)
A: 161 - 340 (UniProt: P13393)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Val 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Arg 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Ser 5 | - | expression tag |
| His 6 | - | expression tag |
| Met 7 | - | expression tag |
| Gly 72 | - | linker |
| Gly 73 | - | linker |
| Gly 74 | - | linker |
| Ser 75 | - | linker |
| Gly 76 | - | linker |
| Gly 77 | - | linker |
| Gly 78 | - | linker |
| Ser 79 | - | linker |
| Gly 80 | - | linker |
| Gly 81 | - | linker |
| Gly 82 | - | linker |
| Ser 83 | - | linker |
| Met 84 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 41 | Gly 41 | engineered mutation |
| Arg 42 | Ala 42 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 30316.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 708.3 | |
| All* | Total formula weight | 31024.6 |
