4AUK

Crystal structure of C2498 2'-O-ribose methyltransferase RlmM from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE M	polymer	375	43124.5	2	UniProt (P0ADR6) Pfam (PF18125) Pfam (PF21239) Pfam (PF01728) In PDB	ESCHERICHIA COLI	23S RRNA (CYTIDINE2498-2'-O)-METHYLTRANSFERASE, 23S RRNA 2'-O-RIBOSE METHYLTRANSFERASE RLMM
2	A, B	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
3	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	A	L(+)-TARTARIC ACID	non-polymer		150.1	1	Chemie (TLA)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
8	B, A	1,2-ETHANEDIOL	non-polymer		62.1	17	Chemie (EDO)
9		water	water		18.0	552	Chemie (HOH)

Sequence modifications

A, B: 1 - 366 (UniProt: P0ADR6)

PDB	External Database	Details
Ser 367	-	expression tag
Lys 368	-	expression tag
Gly 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	86248.9
Non-Polymers*	Number of molecules	30
	Total formula weight	2430.5
All*	Total formula weight	88679.5

*Water molecules are not included.