4ATX

Rigor kinesin motor domain with an ordered neck-linker, docked on tubulin dimer, modelled into the 8A cryo-EM map of doublecortin- microtubules decorated with kinesin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	TUBULIN BETA-2B CHAIN	polymer	445	49907.8	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	BOS TAURUS (CATTLE)
2	B (B)	TUBULIN ALPHA-1D CHAIN	polymer	452	50236.4	1	UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953)	BOS TAURUS (CATTLE)
3	C (C)	KINESIN-1 HEAVY CHAIN	polymer	340	38137.0	1	UniProt (Q2PQA9) Pfam (PF00225)	RATTUS NORVEGICUS (NORWAY RAT)	CONVENTIONAL KINESIN HEAVY CHAIN, UBIQUITOUS KINESIN HEAVY CHAIN, UKHC
4	D (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
5	E (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)

Sequence modifications

A: 1 - 455 (UniProt: Q6B856)

PDB	External Database	Details
Ala 57	Thr 55	conflict
Val 172	Met 170	conflict
Ala 298	Ser 296	conflict
Val 318	Ile 316	conflict

B: 1 - 452 (UniProt: Q2HJ86)

PDB	External Database	Details
Ile 7	Val 7	conflict
Ile 114	Leu 114	conflict
Ser 136	Leu 136	conflict
Val 137	Ile 137	conflict
Gly 265	Ile 265	conflict
Glu 358	Gln 358	conflict
Val 437	Met 437	conflict
Glu 450	Asp 450	conflict

C: 1 - 340 (UniProt: Q2PQA9)

PDB	External Database	Details
Asn 92	Thr 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	138281.1
Non-Polymers*	Number of molecules	2
	Total formula weight	966.4
All*	Total formula weight	139247.5

*Water molecules are not included.