4ATX
Rigor kinesin motor domain with an ordered neck-linker, docked on tubulin dimer, modelled into the 8A cryo-EM map of doublecortin- microtubules decorated with kinesin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TUBULIN BETA-2B CHAIN | polymer | 445 | 49907.8 | 1 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | BOS TAURUS (CATTLE) | |
| 2 | B (B) | TUBULIN ALPHA-1D CHAIN | polymer | 452 | 50236.4 | 1 | UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) | BOS TAURUS (CATTLE) | |
| 3 | C (C) | KINESIN-1 HEAVY CHAIN | polymer | 340 | 38137.0 | 1 | UniProt (Q2PQA9) Pfam (PF00225) | RATTUS NORVEGICUS (NORWAY RAT) | CONVENTIONAL KINESIN HEAVY CHAIN, UBIQUITOUS KINESIN HEAVY CHAIN, UKHC |
| 4 | D (A) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 5 | E (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
A: 1 - 455 (UniProt: Q6B856)
B: 1 - 452 (UniProt: Q2HJ86)
C: 1 - 340 (UniProt: Q2PQA9)
| PDB | External Database | Details |
|---|---|---|
| Ala 57 | Thr 55 | conflict |
| Val 172 | Met 170 | conflict |
| Ala 298 | Ser 296 | conflict |
| Val 318 | Ile 316 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 7 | Val 7 | conflict |
| Ile 114 | Leu 114 | conflict |
| Ser 136 | Leu 136 | conflict |
| Val 137 | Ile 137 | conflict |
| Gly 265 | Ile 265 | conflict |
| Glu 358 | Gln 358 | conflict |
| Val 437 | Met 437 | conflict |
| Glu 450 | Asp 450 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asn 92 | Thr 92 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 138281.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 966.4 | |
| All* | Total formula weight | 139247.5 |
