4ATX
Rigor kinesin motor domain with an ordered neck-linker, docked on tubulin dimer, modelled into the 8A cryo-EM map of doublecortin- microtubules decorated with kinesin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000226 | biological_process | microtubule cytoskeleton organization |
| A | 0000278 | biological_process | mitotic cell cycle |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005200 | molecular_function | structural constituent of cytoskeleton |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005874 | cellular_component | microtubule |
| A | 0007017 | biological_process | microtubule-based process |
| A | 0007399 | biological_process | nervous system development |
| A | 0015630 | cellular_component | microtubule cytoskeleton |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0046982 | molecular_function | protein heterodimerization activity |
| A | 1902669 | biological_process | positive regulation of axon guidance |
| B | 0000226 | biological_process | microtubule cytoskeleton organization |
| B | 0000278 | biological_process | mitotic cell cycle |
| B | 0005200 | molecular_function | structural constituent of cytoskeleton |
| B | 0005525 | molecular_function | GTP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0005874 | cellular_component | microtubule |
| B | 0007017 | biological_process | microtubule-based process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0003777 | molecular_function | microtubule motor activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0007018 | biological_process | microtubule-based movement |
| C | 0008017 | molecular_function | microtubule binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE GDP A 600 |
| Chain | Residue |
| A | GLY10 |
| A | ASP179 |
| A | ASN206 |
| A | TYR224 |
| A | ASN228 |
| A | GLN11 |
| A | CYS12 |
| A | GLN15 |
| A | SER140 |
| A | GLY142 |
| A | GLY144 |
| A | THR145 |
| A | GLY146 |
| site_id | AC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE GTP B 500 |
| Chain | Residue |
| A | LEU248 |
| A | LYS254 |
| B | GLY10 |
| B | GLN11 |
| B | ALA12 |
| B | GLN15 |
| B | ALA99 |
| B | ALA100 |
| B | ASN101 |
| B | SER140 |
| B | GLY143 |
| B | GLY144 |
| B | THR145 |
| B | GLY146 |
| B | ILE171 |
| B | GLU183 |
| B | ASN206 |
| B | TYR224 |
| B | ASN228 |
Functional Information from PROSITE/UniProt
| site_id | PS00227 |
| Number of Residues | 7 |
| Details | TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG |
| Chain | Residue | Details |
| B | GLY142-GLY148 | |
| A | GLY142-GLY148 |
| site_id | PS00228 |
| Number of Residues | 4 |
| Details | TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI |
| Chain | Residue | Details |
| A | MET1-ILE4 |
| site_id | PS00411 |
| Number of Residues | 12 |
| Details | KINESIN_MOTOR_1 Kinesin motor domain signature. GKLyLVDLAGSE |
| Chain | Residue | Details |
| C | GLY225-GLU236 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00283 |
| Chain | Residue | Details |
| C | GLY85 | |
| A | SER140 | |
| A | GLY144 | |
| A | THR145 | |
| A | GLY146 | |
| A | ASN206 | |
| A | ASN228 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0000250|UniProtKB:P33176 |
| Chain | Residue | Details |
| C | ALA2 | |
| B | GLU71 | |
| B | SER140 | |
| B | GLY144 | |
| B | THR145 | |
| B | THR179 | |
| B | ASN206 | |
| B | ASN228 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250|UniProtKB:P33176 |
| Chain | Residue | Details |
| C | LYS213 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q71U36 |
| Chain | Residue | Details |
| B | LYS40 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:P68373 |
| Chain | Residue | Details |
| B | TYR282 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P68373 |
| Chain | Residue | Details |
| B | SER439 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:P68369 |
| Chain | Residue | Details |
| B | GLU446 | |
| A | THR292 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:Q71U36 |
| Chain | Residue | Details |
| B | TYR452 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:Q2T9S0 |
| Chain | Residue | Details |
| A | GLU448 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P07437 |
| Chain | Residue | Details |
| A | LYS60 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P07437 |
| Chain | Residue | Details |
| A | LYS326 |
