4ATU
Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, C, E, G) | TUBULIN BETA-2B CHAIN | polymer | 445 | 49907.8 | 4 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | BOS TAURUS (CATTLE) | |
| 2 | B, D, F, H (B, D, F, H) | TUBULIN ALPHA-1D CHAIN | polymer | 452 | 50236.4 | 4 | UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) | BOS TAURUS (CATTLE) | |
| 3 | I (I) | NEURONAL MIGRATION PROTEIN DOUBLECORTIN | polymer | 373 | 41604.7 | 1 | UniProt (O43602) Pfam (PF03607) | HOMO SAPIENS (HUMAN) | DOUBLIN, LISSENCEPHALIN-X, LIS-X |
| 4 | J, L, N, P (A, C, E, G) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 4 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 5 | K, M, O, Q (B, D, F, H) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
A, C, E, G: 1 - 455 (UniProt: Q6B856)
B, D, F, H: 1 - 452 (UniProt: Q2HJ86)
I: 2 - 360 (UniProt: O43602)
| PDB | External Database | Details |
|---|---|---|
| Ala 57 | Thr 55 | conflict |
| Val 172 | Met 170 | conflict |
| Ala 298 | Ser 296 | conflict |
| Val 318 | Ile 316 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 450 | Asp 450 | conflict |
| Ile 7 | Val 7 | conflict |
| Ile 114 | Leu 114 | conflict |
| Ser 136 | Leu 136 | conflict |
| Val 137 | Ile 137 | conflict |
| Gly 265 | Ile 265 | conflict |
| Glu 358 | Gln 358 | conflict |
| Val 437 | Met 437 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly -12 | - | expression tag |
| Ala -11 | - | expression tag |
| Met -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Ile -1 | - | expression tag |
| Cys 0 | - | expression tag |
| Arg 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 442181.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 3865.5 | |
| All* | Total formula weight | 446046.7 |
