4ATU

Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	TUBULIN BETA-2B CHAIN	polymer	445	49907.8	4	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	BOS TAURUS (CATTLE)
2	B, D, F, H	TUBULIN ALPHA-1D CHAIN	polymer	452	50236.4	4	UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) In PDB	BOS TAURUS (CATTLE)
3	I	NEURONAL MIGRATION PROTEIN DOUBLECORTIN	polymer	373	41604.7	1	UniProt (O43602) Pfam (PF03607) In PDB	HOMO SAPIENS (HUMAN)	DOUBLIN, LISSENCEPHALIN-X, LIS-X
4	A, C, E, G	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
5	B, D, F, H	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)

Sequence modifications

A, C, E, G: 1 - 455 (UniProt: Q6B856)

PDB	External Database	Details
Ala 57	Thr 55	conflict
Val 172	Met 170	conflict
Ala 298	Ser 296	conflict
Val 318	Ile 316	conflict

B, D, F, H: 1 - 452 (UniProt: Q2HJ86)

PDB	External Database	Details
Glu 450	Asp 450	conflict
Ile 7	Val 7	conflict
Ile 114	Leu 114	conflict
Ser 136	Leu 136	conflict
Val 137	Ile 137	conflict
Gly 265	Ile 265	conflict
Glu 358	Gln 358	conflict
Val 437	Met 437	conflict

I: 2 - 360 (UniProt: O43602)

PDB	External Database	Details
Gly -12	-	expression tag
Ala -11	-	expression tag
Met -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Ile -1	-	expression tag
Cys 0	-	expression tag
Arg 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	442181.2
Non-Polymers*	Number of molecules	8
	Total formula weight	3865.5
All*	Total formula weight	446046.7

*Water molecules are not included.