4ATU

Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI TECNAI F20
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2
Detector	KODAK SO-163 FILM

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	8.3
Software	Flex-EM,UCSF Chimera,FREALIGN,SPIDER